双硫腙化学修饰电极溶出伏安法测定人体中痕量铅

我们研制了双硫腙化学修饰电极,并应用于人体中痕量铅的测定。由于该电极电解富集铅具有特效性,因而本法测铅有较高的灵敏度和较好的选择性。检出下限为2.5×10~(-10)mol/L(受空白所限)。实测人体样品,铅的回收率 SD为97.3±5.7％,变异系数CV为5.9％。     

去甲斑蝥素缓释制剂毒性与疗效的实验研究

目的：初步探讨去甲斑蝥素缓释制剂的减毒增效作用。方法：选择泊洛沙姆407作为去甲斑蝥素局部用药的载体,将去甲斑蝥素制成缓释剂型,在初步证实该制剂兔肝内注射后确能缓慢释放的前提下,比较不同剂型的去甲斑蝥素的毒性及对荷W256肿瘤大鼠的抗肿瘤效果。结果：（1）去甲斑蝥素缓释制剂肝内注射后在局部至少能停留4 h;（2）去甲斑蝥素缓释制剂的毒性明显低于单纯去甲斑蝥素;（3）去甲斑蝥素缓释剂型治疗组大鼠肿瘤生长受到显著抑制,肿瘤组织坏死广泛,治疗后平均生存期延长（与单纯去甲斑蝥素组相比,P<0.05）,综合疗效优于单纯去甲斑蝥素组。结论：去甲斑蝥素缓释制剂肝内局部注射能够通过延缓去甲斑蝥素的释放速度,增加其与肿瘤细胞直接作用的时间等途径达到减毒增效的目的。     

Role of ROCK1 in the podocyte injury induced by oxidized low-density lipoprotein

Objective To explore the role of ROCK1 in oxidized low-density lipoprotein (ox-LDL) induced podocyte injury and its possible mechanism. Methods The conditionally immortalized mouse podocyte cells were cultured in vitro and exposed to 20 μg/ml ox-LDL for 24 h. Western blotting was used to analyze the expression level of p-MYPT, nephrin, LC3-Ⅱ, p62, p-ULK1 in groups of control, ox-LDL, ROCK1 siRNA with ox-LDL, wtROCK1 with ox-LDL. Podocytes were incubated with DiI labeled ox-LDL for 4 h and fluorescence microscope was used to analyze lipid distribution. Results Compared with control group, ox-LDL increased cell cholesterol accumulation, activated ROCK along with decreased nephrin, LC3-Ⅱ(P＜0.05), and increased p62, and p-ULK1 expression (P＜0.05). Over-expression of ROCK1 significantly decreased the expression of nephrin and LC3-Ⅱ, but up-regulated the levels of p62, p-ULK1 and cell cholesterol accumulation in ox-LDL stimulated podocytes (P＜0.05). In contrast, Inhibition of ROCK1 protected podocyte by improved lipophagy. Conclusion ROCK1 mediated disfunction of lipophagy contributes to the ox-LDL induced podocyte injury.     

晚期非小细胞肺癌放疗结合热疗对比临床研究

目的 对比研究晚期非小细胞肺癌体外高频HG-2000热疗结合放疗的初步临床探讨.方法 分析2002～2004晚期非小细胞肺癌患者54例,随机分为A、B两组:A组为放疗组;B组为热放综合治疗组.A组27例,采用6MV-X线常规分割放疗或立体适形放疗,照射至肿瘤剂量DT40.0～45.0 Gy时,缩野避开脊髓放疗至肿瘤总剂量DT60～65 Gy ,5～7周完成.B组27例,放疗期间同时结合高频HG-2000体外热疗机增敏热疗,放疗方法及剂量与A组相同.热疗在每次放疗前或放疗后1小时内进行.温度控制在41.5～43℃,肿瘤中心温度45～53℃,60 min/次,2次/周,2次间隔72 h以上,每疗程6～10次.结果 近期疗效:治疗结束后1～3个月以CT检查的影像学结果为依据,判断近期疗效.有效率(CR PR)放疗组与综合组分别是74%、82%;无效率(NC PD)放疗组与综合组分别是6%、0%.生存率:1、2年生存率放疗组和综合组分别为84.5%、50.7%和87.3%、52.3%.中位生存期分别为17.5和18.2个月,2组比较差异无显著性意义(χ2=4.25,P=0.053).毒副反应:皮肤反应,热疗组轻度烫伤2例,Ⅱ度烫伤1例;急性放射性肺炎,放疗组54例,热放组62例.1～2级骨髓抑制,放疗组19.6%,热放组18.8%.结论 高频热疗结合放疗治疗局部晚期非小细胞肺癌疗效优于单纯放疗.放射性肺炎发生率有待进一步探讨.     

补肾中药骨密片防治继发性骨质疏松症实验研究

目的补肾中药用于实验性继发性骨质疏松症模型大鼠,观察其疗效,并对补肾中药的机理做初步的探讨。方法使用糖皮质激素肌肉注射制造继发性骨质疏松症动物模型,同时分别给予维生素Ｄ３、补肾中药“骨密片”治疗３月后,观察模型动物全身、腰椎及股骨骨密度（ＢＭＤ）、三点弯曲试验、血钙、血磷、血ＡＫＰ等理化指标。结果中药及中西医结合治疗组大鼠钙、血磷、血ＡＫＰ等均高于模型组（Ｐ〈０．０５）,腰椎及股骨ＢＭＤ以及三点弯     

住院精神病患者暴力行为危险因素分析与防范对策

目的对住院精神疾病患者暴力行为危险因素进行分析,为预防危险行为的发生提供客观依据。方法收集425例住院精神病患者的相关资料,按有无暴力行为进行统计分析。结果住院精神病患者暴力行为的发生率为29.6%,非自愿入院、既往暴力行为史、未婚或离异、无业以及有幻觉、被控或被害症状为暴力行为危险因素(P<0.05,P<0.01)。结论住院精神病患者暴力行为的发生率较高,影响因素较多。应重视住院患者暴力危险因素的评估,采取针对性措施降低其暴力行为。     

8-羟基喹啉化学修饰电极溶出伏安法测定人体中痕量铜

我们研制了8-羟基喹啉化学修饰电极,并将其应用于人体中痕量铜的分析。由于修饰在电极上的8-羟基喹啉能与铜在一定条件下生成疏水性螯合物,因而能有效地提高电极的选择性畜集效率,使本法具有较高的灵敏度和较好的选择性,方法的检出下限为5×10~(-10)mol/L(受空白所限)。实测人体样品,铜的回收率为94.2±6.0％(±SD),变异系数CV=6.4％。用本法测定了铅蓄电池制造工和对照组的血铜、发铜,获得满意结果。     

Mechanisms of Natural Killer Cell-mediated Tumor Cell Cytolysis at a Single Cell Level

The interaction between natural killer cell and tumor target cells at asingle cell level was investigated.The study with a modified single cell cytotoxicassay showed the heterogeneity and requirment of target cell membrane fluidity inthe formation of natural killer cell-tumor target cell conjugates,as well as aninternal disintegration model of natural killer cell-mediated target cell lysis,which may have some relation to the increased lysosome reaction in damagedtargtet cells as revealed by ultrastructural study.From this finding,a“target cellsuicide”model of natural killer cell mediated target cell lysis is proposed.Inaddition to these findings,the interaction of natural emperipolesis lymphocyteswith tumor cells and its relationship to natural killer cell-mediated killing werealso studied and are discussed here.     

慢性阻塞性肺疾病患者治疗前后血浆基质金属蛋白酶-9及其抑制物-1水平的变化

目的 探讨COPD急性加重期患者治疗前后血浆基质金属蛋白酶-9（MMP-9）及抑制物1（TIMP-1）水平的变化.方法 选择COPD急性加重期患者50例（病例组）,予以吸氧、抗感染、止咳化痰及解痉平喘等常规治疗1周,观察治疗前后血浆MMP-9、TIMP-1水平和MMP-9/TIMP-1比值的变化,观察临床疗效.另选择同期健康体检者30例作为对照组.结果 病例组治疗前血浆MMP-9、TIMP-1水平和MMP-9/TIMP-1比值明显高于对照组（t=2.97、2.44、2.31,P＜0.05）.治疗1周后,病例组血浆TMMP-9、TIMP-1水平和MMP-9/TIMP-1比值较治疗前明显下降（t=2.86、2.39、2.27,P＜0.05）;治疗后临床总有效率为92.0%（46/50）.结论 COPD急性加重期患者血浆MMP-9、TIMP-1水平和MMP-9/TIMP-1比值升高,血浆MMP-9、TIMP-1水平和MMP-9/TIMP-1比值可作为COPD患者病情严重程度和治疗疗效观察指标.     

Oxidized LDL leads to podocyte injury through PTEN/SR-A pathway

Objective To evaluate the effect of oxidized LDL (Ox-LDL) on scavenger receptor A (SR-A) expression in mouse podocytes and to explore the possible mechanism. Methods The conditionally immortalized mouse podocyte cell line was cultured in vitro and exposed to Ox-LDL of different concentrations for 24 h, or 20 mg/L Ox-LDL for different hours. Cell cholesterol accumulation was examined. Real-time quantity PCR and Western blotting were used to analyze the expression level of PTEN, SR-A and nephrin. Podocytes were incubated with DiI labeled Ox-LDL for 4 h and immunofluorescence was used to analyze lipid uptaking. To further confirm the relationship between PTEN and SR-A, PTEN inhibitor bpv (hOpic) [dipotassium bisperoxo (5-hydroxypyridine-2-carboxyl) oxovanadate (V) ], PTEN siRNA and PTEN adenovirus (adPTEN) were used to dual-directional regulate PTEN expression, so as to observe the change of SR-A, nephrin, cell cholesterol accumulation and lipid uptake. Results SR-A was expressed on mouse podocyte and mediated podocyte lipid uptake. Compared with control group, Ox-LDL increased cell cholesterol accumulation, and up-regulated SR-A expression along with inhibited expression of PTEN and nephrin (P＜0.05), which were correlate with dose and exposure time of Ox-LDL. Expression of PTEN significantly inhibits the expression level of SR-A and lipid uptake induced by Ox-LDL (P＜0.05), thereby decreasing cell cholesterol accumulation, but up-regulating nephrin level (P＜0.05). However, down-regulation of PTEN could cause opposite effect. Conclusion Ox-LDL up-regulates SR-A through decreasing the expression of PTEN, and contributes to podocyte injury.